Journal: International journal of molecular sciences

Article Title: eIF4A1 Is a Prognostic Marker and Actionable Target in Human Hepatocellular Carcinoma.

doi: 10.3390/ijms24032055

Figure Lengend Snippet: Figure 5. Human hepatocellular carcinoma cell lines express eIF4A1, eIF4A2, and DDX3 proteins. (A) The levels of the three proteins were assessed in HLE, HLF, PLF/PRF/5, SNU182, and SNU449 cell lines by western blot analysis. GAPDH was used as a loading control, and protein band intensities of eIF4A1, eIF4A2, and DDX3 were normalized to GAPDH levels (B).

Article Snippet: The human HCC cell lines HLE (JCRB0404, Xenotech, Kansas City, MO, USA), HLF (JCRB0405, Tebu-bio GmbH, Offenbach, Germany), PLC/PRF/5 (300315, CLS Cell lines Service GmbH, Eppelheim, Germany), SNU182 (CRL-2235, LGC Standards GmbH), and SNU449 (CRL-2234, LGC Standards GmbH) were cultured at 37 ◦C in a 5% CO2 humidified atmosphere.

Techniques: Western Blot, Control

Journal: Oncology reports

Article Title: Significance of Lysyl oxidase‑like 2 gene expression on the epithelial‑mesenchymal status of hepatocellular carcinoma.

doi: 10.3892/or.2018.6349

Figure Lengend Snippet: Figure 1. Relationship between the expression of LOXL2 and vimentin and E‑cadherin levels in human HCC cell lines with matched EMT. (A) Levels of LOX and LOX family mRNAs in HCC cell lines. (B) The levels of LOXL2, vimentin and E‑cadherin mRNAs in HCC cell lines. A high level of LOXL2 expression was correlated with a high level of vimentin expression and a low level of E‑cadherin expression. HCC, hepatocellular carcinoma.

Article Snippet: Human HCC cell lines (SNU-182, SNU-387, SNU-398, SNU-423 and SNU-449) were obtained from the American Type Culture Collection (ATCC; Rockville, MD, USA).

Techniques: Expressing

Journal: Oncology reports

Article Title: Significance of Lysyl oxidase‑like 2 gene expression on the epithelial‑mesenchymal status of hepatocellular carcinoma.

doi: 10.3892/or.2018.6349

Figure Lengend Snippet: Figure 2. Correlation of LOXL2 mRNA expression in human HCC and normal tissues. HCC, hepatocellular carcinoma.

Article Snippet: Human HCC cell lines (SNU-182, SNU-387, SNU-398, SNU-423 and SNU-449) were obtained from the American Type Culture Collection (ATCC; Rockville, MD, USA).

Techniques: Expressing

Journal: Oncology reports

Article Title: Significance of Lysyl oxidase‑like 2 gene expression on the epithelial‑mesenchymal status of hepatocellular carcinoma.

doi: 10.3892/or.2018.6349

Figure Lengend Snippet: Figure 5. Wound healing and invasion assays in HCC cell lines. (A) BAPN significantly decreased migration in the HLF and SK‑HEP-1 cells (P<0.05). (B) BAPN significantly inhibited invasion in the SK‑HEP-1 cells (P<0.05).

Article Snippet: Human HCC cell lines (SNU-182, SNU-387, SNU-398, SNU-423 and SNU-449) were obtained from the American Type Culture Collection (ATCC; Rockville, MD, USA).

Techniques: Migration

Journal: Oncology reports

Article Title: Significance of Lysyl oxidase‑like 2 gene expression on the epithelial‑mesenchymal status of hepatocellular carcinoma.

doi: 10.3892/or.2018.6349

Figure Lengend Snippet: Figure 4. Proliferation assay in human HCC cell lines. (A and B) In both cell lines, even when the BAPN concentration was increased, the proliferation rate was not affected.

Article Snippet: Human HCC cell lines (SNU-182, SNU-387, SNU-398, SNU-423 and SNU-449) were obtained from the American Type Culture Collection (ATCC; Rockville, MD, USA).

Techniques: Proliferation Assay, Concentration Assay